The RNase detection kit is based on a fluorophore-labeled RNA probe. When the sample does not contain RNase activity, the probe is stable and does not produce a fluorescent signal; when the sample contains RNase activity, the probe is degraded, resulting in a gradual enhanced fluorescence signal; the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em=535/575nm to determine whether the sample is contaminated by RNase.
Application
This kit is used to detect RNase contamination in samples.
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